지방이식 for Dummies
지방이식 for Dummies
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Do there is a protocol or suggested antibodies to execute ICC staining on my entirely differentiated ALI cultures?
CD34+ hematopoietic stem and progenitor cells (HSPCs) Perform a pivotal part in preserving and replenishing the hematopoietic system and maintain substantial possible with the procedure of genetic blood Problems and immunological diseases. The emergence of gene modifying technologies, especially CRISPR-Cas9, has transformed a chance to introduce specific modifications in these cells.
Flow cytometry may be used to measure the performance of differentiation protocols While using the STEMdiff™ system, by checking out precise marker expression at Each and every phase on the differentiation process. It can be used for other applications, which include cell sorting, immunophenotyping, and purity assessment.
If cells tend not to dissociate properly with trituration, select a longer ACCUTASE™ incubation, approximately 12 minutes. If monolayers do not dissociate soon after 12 minutes of ACCUTASE™ incubation and trituration, the differentiation was probably unsuccessful.
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For finest benefits, the whole quantity of cargo extra 지방흡입 shouldn't exceed 10% of the response volume. Lowering the reaction quantity to below 80 µL may bring about decrease editing efficiencies and cell recoveries.
Assess the standing by checking a droplet and Stem cell stopping the dissociation when close to eighty% with the cell suspension is one-celled. Stay clear of over-digestion.
Based on the level of mucus accumulation, a 2nd wash may additionally be demanded. See how a mucus clean is executed in this ALI culture differentiation video (skip to 02:24) >
Yes, you’ll locate the ways to accomplish 지방흡입 an ICC staining on the epithelial cells cultured within the ALI During this protocol. Here's a summary of antibodies which might be utilized 가슴수술 for the characterization of airway cultures:
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Notes: Use pipette ideas to Carefully scratch the bottom of each effectively as a way to carry the monolayer off, then dissociate the cell clumps into one-cell suspension by pipetting up and down five - 10 periods.
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The next fluorochrome-conjugated antibodies and dyes are advised to aid analysis of gene-edited CD34+ HSPCs: